DNA

Part:BBa_K156008

Designed by: George McArthur IV   Group: iGEM08_Virginia   (2008-10-27)


BioBrick placeholder 8 (Prefix - MfeI (NotI) SbfI - Suffix)

This BioBrick Placeholder part is one of a set of eight that represent a new technical standard useful in composite part construction. Flanked by the standard BBa restriction sites, these parts are made of internal restriction sites that are unique but compatible with the standard sites (see below). This allows insertion of a desired part (e.g., RBSs, coding regions) within a previously-made construct.

Compatible sites:

EcoRI - ApoI & MfeI
XbaI & SpeI - AvrII, NheI
PstI - NsiI, SbfI

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 45
    Illegal PstI site found at 38
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 46
    Illegal PstI site found at 38
    Illegal PstI site found at 60
    Illegal NotI site found at 7
    Illegal NotI site found at 29
    Illegal NotI site found at 53
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 46
    Illegal PstI site found at 38
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 46
    Illegal PstI site found at 38
    Illegal PstI site found at 60
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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